RESUMO
INTRODUCTION: Salmonella enterica serovar Panama belongs to the D1 serogroup and is frequently associated with nontyphoidal salmonellosis in humans. This study aimed to characterize isolates collected from Northeast Brazil by phenotypic and molecular methods. METHODS: Forty four S. Panama strains were examined for antimicrobial susceptibility, virulence genes, and pulsed field gel electrophoresis (PFGE) types. RESULTS: All strains were susceptible to antibiotics (except for streptomycin), presented classical virulence factors, and could be clustered into four groups and 18 pulsotypes. CONCLUSIONS: This work calls for continuous surveillance for the emergence of antibiotic resistance and new clones in a geographical area.
Assuntos
Salmonella enterica/genética , Fatores de Virulência/genética , Animais , Antibacterianos/farmacologia , Brasil , Farmacorresistência Bacteriana Múltipla , Eletroforese em Gel de Campo Pulsado , Variação Genética , Humanos , Testes de Sensibilidade Microbiana , Salmonella enterica/efeitos dos fármacos , Salmonella enterica/isolamento & purificação , Salmonella enterica/patogenicidadeRESUMO
Abstract INTRODUCTION Salmonella enterica serovar Panama belongs to the D1 serogroup and is frequently associated with nontyphoidal salmonellosis in humans. This study aimed to characterize isolates collected from Northeast Brazil by phenotypic and molecular methods. METHODS Forty four S. Panama strains were examined for antimicrobial susceptibility, virulence genes, and pulsed field gel electrophoresis (PFGE) types. RESULTS All strains were susceptible to antibiotics (except for streptomycin), presented classical virulence factors, and could be clustered into four groups and 18 pulsotypes. CONCLUSIONS This work calls for continuous surveillance for the emergence of antibiotic resistance and new clones in a geographical area.
Assuntos
Animais , Salmonella enterica/genética , Fatores de Virulência/genética , Variação Genética , Brasil , Testes de Sensibilidade Microbiana , Eletroforese em Gel de Campo Pulsado , Salmonella enterica/isolamento & purificação , Salmonella enterica/efeitos dos fármacos , Salmonella enterica/patogenicidade , Farmacorresistência Bacteriana Múltipla , Antibacterianos/farmacologiaRESUMO
Salmonella infections usually occur as gastroenteritis that is generally self-limited. However, some serotypes of Salmonella can cause severe extra-intestinal infections, such as bacteremia and meningitis. Here, we report the first Salmonella Panama case of meningitis in 4-month-old male newborn in Brazil. The invasive strain isolated was susceptible to all antimicrobial agents tested. The genes agfA, fimA, invA, sfbA, phoP, and slyA were detected using polymerase chain reactions. These findings are relevant and physicians should be alert to the possibility of meningitis in newborns due to S. Panama, which can present a high rate of mortality or recurrence of infection.
Assuntos
Meningites Bacterianas/microbiologia , Salmonella enterica/genética , Brasil/epidemiologia , Pré-Escolar , Humanos , Masculino , Meningites Bacterianas/diagnóstico , Meningites Bacterianas/epidemiologia , Reação em Cadeia da Polimerase , Salmonella enterica/isolamento & purificação , SorotipagemRESUMO
Abstract Salmonella infections usually occur as gastroenteritis that is generally self-limited. However, some serotypes of Salmonella can cause severe extra-intestinal infections, such as bacteremia and meningitis. Here, we report the first Salmonella Panama case of meningitis in 4-month-old male newborn in Brazil. The invasive strain isolated was susceptible to all antimicrobial agents tested. The genes agfA, fimA, invA, sfbA, phoP, and slyA were detected using polymerase chain reactions. These findings are relevant and physicians should be alert to the possibility of meningitis in newborns due to S. Panama, which can present a high rate of mortality or recurrence of infection.
Assuntos
Humanos , Masculino , Meningites Bacterianas/microbiologia , Salmonella enterica/genética , Brasil/epidemiologia , Sorotipagem , Reação em Cadeia da Polimerase , Meningites Bacterianas/diagnóstico , Meningites Bacterianas/epidemiologia , Salmonella enterica/isolamento & purificaçãoRESUMO
INTRODUCTION: In December 2001, an outbreak of foodborne gastroenteritis infected 114 of 161 people who ate at a restaurant in Aracaju, State of Sergipe, Brazil. METHODS: The epidemiological and microbiological aspects of the outbreak were characterized. RESULTS: Potato salad made with homemade mayonnaise and stored at unsuitable temperatures was associated with increased risk of foodborne infection. Salmonella Enteritidis was isolated from the diarrheal stools of the hospitalized patients, and genotyping of the fecal samples generated identical randomly amplified polymorphic deoxyribonucleic acid (DNA) profiles. CONCLUSIONS: To the best of our knowledge, this is the first and the only record of a gastrointestinal outbreak in Sergipe.
Assuntos
Surtos de Doenças , Gastroenterite/epidemiologia , Restaurantes , Intoxicação Alimentar por Salmonella/epidemiologia , Salmonella enteritidis/genética , Brasil/epidemiologia , DNA Bacteriano/análise , Fezes/microbiologia , Gastroenterite/microbiologia , Genótipo , Humanos , Técnica de Amplificação ao Acaso de DNA Polimórfico , Inquéritos e QuestionáriosRESUMO
Uma avaliação da magnitude das infecções hospitalares em grandes queimados da área pediátrica do Hospital João Alves Filho foi realizada de 20 de novembro de 1996 a abril de 1997. Neste período foram analisados 74 casos de crianças com idades entre 0 e 12 anos, objetivando verificar a ocorrência de infecções hospitalares (IH), determinar os agentes etiológicos e respectivas sensibilidade e resistência a antimicrobianos. Uma busca aos prontuários forneceu informações sobre os antibióticos empregados como medidas profiláticas. Foi feita análise microbiológica das superfícies do ambiente. Dos 74 pacientes admitidos, 4 (5,4%) apresentaram 14 episódios de infecções nosocomiais (18,97%) causadas por: Pseudomonas aeruginosa (54,14%), Staphylococcus aureus (7,14%), Cedecea lapagei (7,14%), Klebsiella pneumoniae (7,14%), Proteus mirabilis (14,28%) e Proteus vulgaris (7,14%). O patógeno prevalente também nas superfícies, Pseudomonas sp., apresentou sensibilidade a Imipenem (75%), Cefepime, Norfloxacin e Azetreonam (62,5% cada). Os dados indicaram o uso de profiláticos em 100% dos casos
